HiSeq 2000 Compatible Products

Cluster Generation

Cluster the flow cell on the cBot 2 System or cBot System before sequencing on the HiSeq 2000 System.

Sequencing Primers for Dual-Indexed Libraries

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2000:

  • Any Nextera libraries with any number of indexes. Use the single-read (SR) box with SR flow cells and the paired-end (PE) box with PE flow cells.
  • TruSeq HT libraries when performing a dual-indexed run on an SR flow cell.

For more information, see HiSeq 2000 Primers.

Run Components

Use the compatible version of HiSeq Control Software (HCS) and Real-Time Analysis (RTA) software for your kit. For kit details, see HiSeq 2000 Reagent Kits.

Reagent Kits
Cluster Kit SBS Kit HiSeq Software
HiSeq Cluster Kit v4 HiSeq SBS Kit v4 HCS v2.2/RTA v1.18, or later
TruSeq Cluster Kit v3 TruSeq SBS Kit v3 HCS v1.4/RTA v1.12, or later
Additional Primers
Kit cBot Software Version HiSeq Software
TruSeq Dual Index Sequencing Primer Box cBot Software v1.4.36, or later
cBot Recipe Installer v1.0.13
HCS v1.5/RTA v1.13, or later