HiSeq 2500 Support
Performance Specifications
Approximate Run Duration
The duration of a sequencing run depends on read length and version of instrument control software.
|
Read Length |
HCS v2.0 and later with Flow Cell v3 |
HCS v2.0 and later with Rapid Flow Cell |
|
2 x 101 Cycles |
Approx. 10 days |
Approx. 27 Hours |
|
2 x 51 Cycles |
Approx. 5 days |
Approx. 16 Hours |
System Performance
| High Output Run Mode* | Rapid Run Mode* | |||||
|---|---|---|---|---|---|---|
| Read Length | Dual Flow Cell (HiSeq 2500 only) |
Single Flow Cell (HiSeq 1500 or 2500) |
Dual Flow Cell Run Time | Dual Flow Cell (HiSeq 2500 only) |
Single Flow Cell (HiSeq 1500 or 2500) |
Dual Flow Cell Run Time |
| 1 x 36 | 95-105 Gb | 47-52 Gb | 2 days | 18-22 Gb | 9-11 Gb | 7 hr |
| 2 × 50 | 270-300 Gb | 135-150 Gb | 5.5 days | 50-60 Gb | 25-30 Gb | 16 hr |
| 2 x 100 | 540-600 Gb | 270-300 Gb | 11 days | 100-120 Gb | 50-60 Gb | 27 hr |
| 2 x 150 | N/A | N/A | N/A | 150-180 Gb | 75-90 Gb | 40 hr |
| Reads Passing Filter |
Up to 3 billion single reads or 6 billion paired-end reads | Up to 1.5 billion single reads or 3 billion paired-end reads | Up to 600 million single reads or 1.2 billion paired-end reads | Up to 300 million single reads or 600 million paired-end reads | ||
| Quality | Greater than 85% of bases above Q30 at 2 × 50 bp Greater than 80% of bases above Q30 at 2 × 100 bp |
Greater than 85% of bases above Q30 at 2 × 50 bp Greater than 80% of bases above Q30 at 2 × 100 bp Greater than 75% of bases above Q30 at 2 × 150 bp |
||||
*Install specifications based on Illumina PhiX control library at supported cluster densities (between 610-678 K clusters/mm2 passing filter using TruSeq v3 Kits or 700-820 clusters/mm2 passing filter using TruSeq Rapid Kits. Run times for rapid run mode correspond to on-board cluster generation (1.5 hr) and sequencing; for high output mode, run times correspond to sequencing only. Performance may vary based on sample quality, cluster density, and other experimental factors. Early HiSeq 2000 instruments will run slightly slower when upgraded to a HiSeq 2500.
Quality Scores
|
Read Length |
Bases Higher than Q30 |
|
2 x 101 Cycles |
80% |
|
2 x 51 Cycles |
85% |
A quality score (Q-score) is a prediction of the probability of an error in base calling.
|
Q-Score |
Chance of Wrong Base Call |
|
Q40 |
1 error in 10,000 |
|
Q30 |
1 error in 1,000 |
|
Q20 |
1 error in 100 |
|
Q10 |
1 error in 10 |