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The Off-Line Basecaller (OLB) performs base calling and bcl to qseq conversion for the HiSeq, HiScan-SQ, or Genome Analyzer.
The standard workflow is to perform base calling using on-instrument real time analysis (RTA), after which CASAVA performs alignment using the base calling results. If needed, OLB provides the option to perform primary data analysis off-line. In addition, OLB allows you to convert per-cycle base call files (*.bcl) into per read base call files (*_qseq.txt).