Questions & Answers

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Questions & Answers

  • What is the throughput of the BeadXpress Reader?

    Throughput is dependent upon the level of multiplexing and whether you are running a single-color or dual-color detection scanner. Typical throughputs are:

    Multiplex

    Single-Color Detection

    Dual-Color Detection

    10

    140 samples/hr

    120 samples/hr

    96

    90 samples/hr

    68 samples/hr

    384

    44 samples/hr

    30 samples/hr

  • How do you achieve multiplexing with the VeraCode Beads?

    Multiplexing is achieved by pooling together beads with unique codes. Illumina offers standard products to enable users to develop anything from a single-plex to several hundred-plex reactions per sample in a single well.

  • How are the VeraCode Carboxyl Beads used?

    Carboxyl beads have a carboxylated surface, enabling development of protein-based assays. To achieve multiplexing, pool together different tubes of uniquely coded carboxyl beads after immobilizing with proteins of interest.

  • What is Illumina's current regulatory status?

    For information on Illumina's regulatory, quality, and instrument safety certifications, including ISO certifications, go to the Regulatory and Quality Information page.

  • How are the VeraCode Universal Beads used?

    Each uniquely coded VeraCode Universal Bead has a unique oligonucleotide capture sequence attached and can be used to design nucleic-acid based assays. For example, to develop a 3-plex reaction using a single-color detection assay, such as Allele Specific Primer Extension (ASPE), pool together six different tubes of unique VeraCode Universal Oligo Beads (one bead type per allele).

  • Can you tell me about the VeraCode Microbeads?

    The VeraCode technology is based on cylindrical glass microbeads measuring 240 μm in length × 28 μm in diameter. Illumina uses a proprietary technology to inscribe digital holographic elements within each bead. When a laser beam shines through the bead, the holographic elements diffract the light, creating a code image. Each different bead type contains a unique code that can be used to represent information such as the target of interest in multiplex assays. It can also be used to track critical information, including sample ID, laboratory ID, reagent lots, etc. The high-density codes (24 bit) offer a virtually unlimited number of unique bead types.

  • What types of assays can be developed with the VeraCode Beads?

    The glass surface of the VeraCode beads make them ideal for a number of bioassays, including genotyping, gene expression, methylation, and protein-based assays. Solution-based assays, in conjunction with microarrays, can also be developed.