System Liquid
Make sure that sufficient system liquid is in the system liquid
carboy and that the system liquid is clean.
- Always confirm that the intake tubing is below the system
liquid line in the carboy
- Fill the carboy frequently to
prevent the risk of the fluid level running too low
- Keep
the carboy opening covered to prevent contamination from entering
the system liquid
- Periodically empty and clean the carboy
as the interior surfaces can become moldy over time
Priming
Make sure that the fluidics lines are fully primed before use.
Fully primed lines are especially important after periods of inactivity.
- Conduct a tip bleach wash followed by a minimum of 3 Sys Wash
routines before use. Additional Sys Wash routines can be run as
desired
- Watch for excessive bubbles in the syringes during
pipetting steps. If bubbles are present, repeat additional Sys Wash
routines
- If the system liquid carboy runs dry, conduct
repeated Sys Wash routines to prime the fluidics lines after
refilling the carboy
Bleach Washing
Perform periodic bleach washes to minimize potential growth in
fluidics lines and to clean the tips.
- Perform a tip bleach wash (Robot QC Tasks > Robot Bleach
Wash > Option 0) daily before each use. Immediately remove the
bleach trough from the robot deck after use to help eliminate bleach
fumes
- Do not perform a system bleach wash.
Water Circulator for TeFlow
Check for proper fluid levels in the water circulator, which
controls the temperature of the TeFlow unit.
- Before each use, open the lid and make sure that water is
above the heating coils. If the fluid level is low, replenish as
necessary with water prepared in a 1:200 dilution of anti-microbial
additive. It is not necessary to drain the existing fluid
- Confirm that the lid is securely in place to minimize
evaporation
For full details on any of the above items, consult the Infinium Assay Lab Setup and Procedures
Guide document available on our website.