05/15/20
All libraries prepared with the current Illumina library preparation kits not requiring a custom sequencing primer are compatible with all Illumina sequencing platforms.
Sequencing libraries prepared with non-Illumina library preparation methods may require additional optimization on different sequencing platforms. Consider the following differences:
Primer Binding | Deblocking |
Nucleotide Incorporation |
|
---|---|---|---|
iSeq 100 | 65°C | 63°C | 65°C |
MiniSeq | 60°C | 60°C | 60°C |
MiSeq | 65°C | 60°C | 65°C |
NextSeq 500/550 | 60°C | 60°C | 60°C |
NextSeq 1000/2000 | 60°C | 60°C | 60°C |
HiSeq Rapid Run | 65°C | 60°C | 65°C |
HiSeq High Output v3 | 60°C | 55°C | 55°C |
HiSeq High Output v4 | 60°C | 55°C | 65°C |
HiSeq 3000/4000* | 60°C (R1), 65°C | 55°C | 60°C |
NovaSeq | 60°C | 55°C | 60°C |
*HiSeq 3000/4000: cBot Read 1 (R1) primer binding at 60°C. Index and Read 2 primers bind at 65°C