Approximate sizes of sequencing run output folders

04/15/20


Important Note: The run folder sizes listed in this bulletin should be used only as an approximation. Folder size will vary depending on the raw cluster density and the number of clusters passing filter. Folder sizes are based on runs with optimal cluster densities for each platform and dual indexing. For runs on non-patterned flow cells, the size approximations are based on runs with pass filter greater than 80%.

Additional run folder size considerations:

  • iSeq™ 100 folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow
  • MiniSeq™, NextSeq™ 500/550 (NextSeq Control Software v4.0 and higher) folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow
  • MiSeq™ folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow and MiSeq (MiSeq Control Software 3.1 and higher) Local Run Manager FASTQ workflow
  • HiSeq™ 1500/2500, HiSeq 3000/4000, HiSeq X, and NovaSeq™ 6000 folders do not include FASTQ files as part of the total output folder size
  • Illumina recommends storing the output folder for iSeq 100, NextSeq, HiSeq 1500/2500, HiSeq™ 3000/4000, HiSeq X runs on a network location. NovaSeq Control Software requires the output folder to be stored on a network location
  • Folder sizes for HiSeq™ 1500/2500, HiSeq 3000/4000, HiSeq X, and NovaSeq™ 6000 are representative of a single flow cell

iSeq 100

Read Length Output Folder Size in GB
2 x 150 1.5 - 2.5

 

MiniSeq

Read Length Output Folder Size in GB
Mid Output High Output
2 x 150 2.3 - 2.6 7 - 8
2 x 75 N/A 6 - 8
1 x 75 N/A 2 - 4

 

MiSeq

v3 Chemistry
Read Length Output Folder Size in GB
2 x 300 22 - 26
2 x 75 19 - 22

 

v2 Chemistry
Read Length Reagent Kit
Configuration
Output Folder Size in GB
2 x 250 Standard 22 - 24
2 x 250 Nano 1.6 - 1.8
2 x 150 Standard 16 - 18
2 x 150 Micro 3 - 5
2 x 150 Nano 0.8 - 1.2
2 x 25 Standard 14 x 16

 

NextSeq 500/550

Read Length Output Folder Size in GB
Mid Output High Output
2 x 150 62 - 66 160 - 170
2 x 75 39 - 46 92 - 97
1 x 75 N/A 50 - 78

 

HiSeq 1500/2500

Read Length High Output TruSeq SBS v3
Output Folder Size in GB
High Output SBS v4
Output Folder Size in GB
HiSeq Rapid Run v2
Output Folder Size in GB
2 x 250 N/A N/A 80 - 100
2 x 125 295 - 310 190 - 210 N/A
2 x 100 225 N/A 55 - 60
2 x 50 133 77 - 83 32 - 36

 

HiSeq 3000/4000

Read Length Output Folder Size in GB
2 x 150 560
2 x 75 290
2 x 25 80

 

HiSeq X

Read Length Output Folder Size in GB
2 x 150 560

 

NovaSeq

Read Length SP Flow Cell
Output Folder Size in GB
S1 Flow Cell
Output Folder Size in GB
S2 Flow Cell
Output Folder Size in GB
S4 Flow Cell
Output Folder Size in GB
2 x 150 185 370 730 2190
2 x 250 400 N/A N/A N/A