Approximate sizes of sequencing run output folders

The run folder sizes listed in this bulletin should be used only as an approximation. Folder size will vary depending on the raw cluster density and the number of clusters passing filter. Folder sizes are based on runs with optimal cluster densities for each platform and dual indexing. For runs on nonpatterned flow cells, the size approximations are based on runs with pass filter greater than 80%.

Additional run folder size considerations:

• iSeq 100 folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.

• MiniSeq, NextSeq 500/550 (NextSeq Control Software v4.0 and higher) folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.

• MiSeq folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow and MiSeq (MiSeq Control Software 3.1 and higher) Local Run Manager FASTQ workflow.

• NextSeq 1000/2000 folder sizes include local analysis output, which can add another 25-120+ GB depending on workflow.

• HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X, and NovaSeq 6000 folders do not include FASTQ files as part of the total output folder size.

• Folder sizes for HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X, and NovaSeq 6000 are representative of a single flow cell.

Illumina recommends storing the output folder for iSeq 100, NextSeq 500/550, NextSeq 1000/2000, HiSeq 1500/2500, HiSeq 3000/4000, and HiSeq X runs on a network location. NovaSeq Control Software requires the output folder to be stored on a network location.

iSeq 100

Read Length	Output Folder Size in GB
2 x 150	1.5 - 2.5

MiniSeq

Mid Output

Read Length	Output Folder Size in GB
2 x 150	2.3 - 2.6

High Output

Read Length	Output Folder Size in GB
2 x 150	7.0 - 8.0
2 x 75	6.0 - 8.0
1 x 75	2.0 - 4.0

Rapid

Read Length	Output Folder Size in GB
1 x 100	2

MiSeq

v3 chemistry

Read Length	Output Folder Size in GB
2 x 300	22 - 26
2 x 75	19 - 22

v2 chemistry

Read Length	Reagent Kit Configuration	Output Folder Size in GB
2 x 250	Standard	22 - 24
2 x 250	Nano	1.6 - 1.8
2 x 150	Standard	16 - 18
2 x 150	Micro	3.0 - 5.0
2 x 150	Nano	0.8 - 1.2
2 x 25	Standard	14 - 16

NextSeq 500/550

Mid Output

Read Length	Output Folder Size in GB
2 x 150	62 - 66
2 x 75	39 - 46

High Output

Read Length	Output Folder Size in GB
2 x 150	160 - 170
2 x 75	92 - 97
1 x 75	50 - 78

NextSeq 1000/2000

P1

Read Length	Output Folder Size in GB
2 x 300	85-95
2 x 150	50 - 55
2 x 101	40 - 45
2 x 75	35 - 40

P2

Read Length	Output Folder Size in GB
2 x 300	140-160
2 x 150	89 - 99
2 x 101	65 - 66
2 x 75	44 - 55

P3

Read Length	Output Folder Size in GB
2 x 150	165 - 170
2 x 101	103 - 126

NovaSeq 6000

SP

Read Length	Output Folder Size in GB
1 x 36	20
2 x 150	185
2 x 250	400

S1

Read Length	Output Folder Size in GB
2 x 150	370

S2

Read Length	Output Folder Size in GB
2 x 150	730

S4

Read Length	Output Folder Size in GB
1 x 36	240
2 x 150	2190

NovaSeq X/X Plus

10B

Read Length	cBCL Only in GB	BCL Convert (GZIP) in GB	BCL Convert (ORA) in GB
2x50	340 - 570	830 - 1380	540 - 890
2x100	680 - 1130	1650 - 2760	1080 - 1790
2x150	1020 - 1700	2480 - 4140	1620 - 2680

25B

Read Length	cBCL Only in GB	BCL Convert (GZIP) in GB	BCL Convert (ORA) in GB
2x150	2550 - 4250	6200 - 10350	4050 - 6700

The output folder size in these tables is reported in Gigabytes (GB).

For any feedback or questions regarding this article (Illumina Knowledge Article #1508), contact Illumina Technical Support [e-mail].