Approximate sizes of sequencing run output folders

08/10/18


Important Note: The run folder sizes listed in this bulletin should be used only as an approximation. Folder size will vary depending on the raw cluster density and the number of clusters passing filter. Folder sizes are based on runs with optimal cluster densities for each platform and dual indexing. For runs on non-patterned flow cells, the size approximations are based on runs with pass filter greater than 80%.

Additional run folder size considerations:

  • iSeq100 folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.
  • MiniSeq folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.
  • MiSeq folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow.
  • NextSeq, HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X and NovaSeq folders do not include FASTQ files as part of the total output folder size.
  • Illumina recommends storing the output folder for NextSeq, HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X runs on a network location. NovaSeq Control Software requires the output folder to be stored on a network location.
  • Folder sizes for HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X and NovaSeq are representative of a single flow cell.

iSeq100

MiniSeq

MiSeq

NextSeq

HiSeq 1500/2500

HiSeq 3000/4000

HiSeq X

NovaSeq