Successful data transfer from the instrument to a network output
location requires the instrument control software and Local Run
Manager™ (LRM) to be configured according to Windows authentication requirements:
- The local user account (eg, sbsuser) must have read and write
access to the network output folder.
- As best practice, the
folder should be accessed through the Universal Naming Convention
(UNC) path (not a mapped letter drive) and the output folder should
be two levels deep (eg, \\server\level1\level2\).
- If the
network location is on the same domain (see Appendix) as the instrument, user credentials
should be added through Local Run Manager (see Related Support
- If the network location is on a different domain
(see Appendix), see How to add credentials
when the instrument is off domain.
- If the Windows account
passwords change, the credential configurations will also have to be
Requirements for network connectivity
- Use the full UNC path to the output folder. A UNC path uses
double backslashes to precede the name of a computer or server:
- If the output path is only one
level deep, a trailing backslash is required: \\server\level1\.
- Do not use a mapped network drive path (such as Z:\).
- The account must have read/write access to the network
- The account must have at least “read
only” or “list folder content” permissions to all parent folders
within the UNC path. In the example of \\server\level1\level2, the
account must have access to the “level1” folder.
network location needs enough storage for the run. The amount of
storage needed can vary by flow cell type and instrument. Reference
the bulletin Approximate
sizes of sequencing run output folders for estimates.
How to access the full UNC path?
- Launch the Windows Command Prompt app
- Enter the
- This will
display the full UNC path
How to add credentials when the instrument is off domain
- Add the network
credentials for the user account
Prerequisite: Add sbsuser (or
local instrument user) to the server share config (eg, Samba)
- Log in to the instrument with the local user account
(by default, this account is sbsuser for all Illumina
- Open File Explorer and enter the
output network location (in UNC format) into the address
- Enter the credentials of the user who has access to
the desired network location into the Windows Security prompt.
The credentials should be domain\username and the password of
the network storage location.
- Select Remember my
credentials, then select OK.
Figure 1. Windows Security Prompt where credentials are
- Set up Illumina Windows Services
- Search for Services in the taskbar and open the app
with right-click and Run as administrator.
Figure 2. Running Windows Services as administrator.
- Enter the local admin credentials (by default the
credentials will be the sbsadmin user credentials) in the
- Locate the Illumina services.
- Right-click on Illumina Local Run Manager Analysis
- Select Properties and navigate to the
Log On tab.
Figure 3. Selecting Properties for an Illumina
Universal Copy Service.
- Select This account and
browse to search for the account. In the Select User
window, select Advanced and then Find Now. Select
the Local user credentials (default sbsuser) from
the search results.
Figure 4. Selecting the local user credentials after
clicking Find Now.
- Select OK to
acknowledge both windows.
- Enter the Local user
credentials (default sbsuser) password, select
Apply and OK.
- Restart the service.
- Repeat steps 4 - 8 for Illumina Local Run Manager Job
Service, Illumina Universal Copy Service (for
NextSeq 500/550, NextSeq 550Dx, MiniSeq Control Software v2, and
iSeq 100), and Illumina Run Copy Service (for MiniSeq
Control Software v1.1).
- Ensure Internet
Information Services (IIS) is configured for LocalSystem
- Search for Control Panel in the
taskbar, select Administrative Tools and then select
Internet Information Services (IIS) Manager.
- Under the Connections panel on the left side, select the
right arrow to expand the instrument icon, then select
Figure 5. Selecting Application Pools in Internet
Information Services (IIS) Manager.
Local Run Manager and select Advanced
- Confirm that Identity is configured
Figure 6. Confirm that Identity is configured for
- If Identity is NOT configured
for LocalSystem, select Identity, then select the […]
- In Application Pool
Identity, select Built-in account and select
LocalSystem from the dropdown menu.
Figure 7. Ensure Local Run Manager is configured for
- On the left side of the Internet
Information Services (IIS) window, expand Sites by
clicking the caret to the left.
Figure 8. Select the caret to the left of the
Sites folder to expand it.
- Right-click Local
- Select Manage Website, then
select Restart to restart the website.
Note that for NextSeq 550Dx instruments, the credentials need to be
added for both Dx and RUO modes if the output folder is located on a
network location for both modes.
Configuring output folder to the network
Instrument Control Software
- Set Output Folder location as full UNC path with trailing
Figure 9. Setting the Output Folder location in the
instrument control software.
Local Run Manager
- Refer to the bulletin Local
Run Manager: How to requeue and import run data for reanalysis
and the section To import data to a new analysis module.
Import a run to be able to set the Base Output Folder to the network
location. Enter the network location as the full UNC path.
Figure 10. Setting the network location (using full UNC path)
in Local Run Manager when importing a run.
Changing Windows Account Passwords
If the Windows account passwords change, the credential
configurations will also need to be updated. This process might
include updating the user credentials for the Illumina Windows
Services. Failure to update the password can cause connectivity issues
and failures during sequencing runs.
Appendix: How to determine if instrument
is ON or OFF Domain.
- Open Windows Control Panel using Win+x short cut on the
- From Windows Control Panel, select
- Under Computer name, domain, and work group
settings, determine whether the system is off domain (Workgroup) or
on Domain as illustrated in Figure 11.
Figure 11. Domain Settings are found under System in Control
Related Support Bulletins