NextSeq 500/550 instruments with NextSeq Control Software (NCS) v3.0.0/v4.0.0 or NextSeq Operating Software (NOS) v1.2/v1.3 run Windows 10 Operating System (OS), which requires updating the password every 180 days by default. When prompted, as shown in Figure 1a, select OK, then update the Windows password, as shown in Figure 1b.
Figure 1a. Password update prompt.
Figure 1b. Password update.
Changing the Windows 10 OS password does not automatically update passwords used for Universal Copy Service (UCS) and Local Run Manager (LRM). These programs are used by the NextSeq 500/550 system to transfer sequencing run data, for run set-up, and for data analysis. This bulletin provides instructions for updating the UCS and LRM passwords following the Windows 10 OS password update on systems running NextSeq NCS v3.0.0/v4.0.0 and NOS v1.2/v1.3.
If the UCS and LRM service credentials are not updated, UCS and LRM service initiation and operation may be disabled. Disabled operation prevents new run settings from being entered and data transfer from completing. NCS/NOS may fail to initialize, or the following errors may occur:
First, to confirm which software version is running, in the control software Home screen select Manage Instrument, then About. The NCS System Suite Version is listed on the About page.
Next, it is important to determine if the NextSeq 500/550 instrument is on domain or off domain, as different credential updating procedures are used for different system IT settings. Consult your IT department or refer to the Appendix for help with determining if the NextSeq 500/550 system is on domain (Section I) or off domain (Section II).
Section I: On domain NextSeq 500/550 systems - Update UCS and LRM credential via service accounts setting in LRM
Figure 2. System Settings in LRM dashboard navigation bar.
Figure 3. Service Accounts tab in LRM.
Figure 4. Windows Services credentials confirmation.
For this example, in Windows Services, credentials are set to the local NextSeq 500/550 account (.\sbsuser).
Section II: Off domain NextSeq 500/550 systems - Update UCS and LRM credentials manually via Windows Services
For off domain NextSeq 500/550 systems with NCS v3.0.0/v4.0.0 and easier credentialing, Illumina recommends upgrading to NCS v4.0.2 and following instructions in Section I of bulletin How to update Windows 10 OS credentials for Universal Copy Service and Local Run Manager for platforms with NextSeq Control Software (NCS) v4.0.1. or later, MiniSeq Control Software (MNCS) v2 or later, MiSeq Control Software (MCS) v4 or later. To update credentials in NCS v3.0.0/v4.0.0 and NOS v1.2/v1.3, see the following instructions:
Figure 5. In Windows Services, select Run as administrator..
Figure 6. Selecting Properties for Illumina Universal Copy Service.
Figure 7. Selecting the local user credentials after clicking Find Now.
Figure 8. Example of Output Network Location.
Figure 9. Windows Security Prompt where credentials are entered.
NOTE: Each time the Windows 10 OS password is updated, the UCS/LRM Services credentials also need to be updated. Contact email@example.com for further assistance.
For more detailed instructions, first-time setup, or to update the Internet Information Services (IIS) LRM user credentials for importing runs, refer to the support bulletin How to connect benchtop instruments and Local Run Manager to a network location.
Appendix: How to determine if instrument is on domain.
Figure 10. Domain Settings are found under System in Control Panel.
Related Support Bulletins