NextSeq 500/550 instruments with NextSeq Control Software (NCS) v4.0.1 or later, MiniSeq Control Software (MNCS) v2 or later, and MiSeq Control Software (MCS) v4 or later run Windows 10 Operating System (OS). Windows 10 OS requires updating the password every 180 days by default. When prompted, as shown in Figure 1a, select OK, then update the Windows password, as shown in Figure 1b.
Figure 1a. Password update prompt.
Figure 1b. Password update.
Changing the Windows 10 OS password does not automatically update passwords used for Universal Copy Service (UCS) and Local Run Manager (LRM) services. UCS and LRM are used by the NextSeq 500/550, MiniSeq, and MiSeq systems to transfer sequencing run data, for run set-up, and for data analysis. This bulletin provides instructions for updating UCS and LRM passwords following the Windows 10 OS password update when running NCS v4.0.1 or later, MNCS v2 or later, and MCS v4 or later.
If the UCS and LRM service credentials are not updated, UCS and LRM service initiation and operation may be disabled. Disabled operation prevents entry of new run settings and completion of data transfer. The control software may fail to initialize, or the following errors may occur:
First, to confirm which software version is running, in the control software Home screen select Manage Instrument, then About. The System Suite Version is listed on the About page.
If the NextSeq 500/550 system is running NCS v4.0.0/v3.0.0 or NOS v1.2/v1.3, reference the support bulletin How to update Windows 10 OS credentials for Universal Copy Service and Local Run Manager for platforms with NextSeq Control Software (NCS) v4.0.0/v3.0.0 or NextSeq Operating Software (NOS) v1.2/v1.3..
Update UCS and LRM credentials from Service Accounts setting in LRM:
Figure 2. System Settings in LRM dashboard navigation bar.
Figure 3. Service Accounts tab in LRM.
Figure 4. Windows Services credentials confirmation.
For this example, in Windows Services, credentials are set to the local instrument account (.\sbsuser).
Figure 5. Example of Output Network Location.
Figure 6. Windows Security Prompt where credentials are entered.
NOTE: Each time the Windows 10 OS password is updated, the UCS/LRM Services credentials also need to be updated. Contact email@example.com for further assistance.
For more detailed instructions, first-time setup, or to update the Internet Information Services (IIS) LRM user credentials for importing runs, refer to the support bulletin How to connect benchtop instruments and Local Run Manager to a network location.
Figure 7. Domain Settings are found under System in Control Panel.
Related Support Bulletins