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Create an AmpliSeq for Illumina Hotspot Design/Configure an AmpliSeq for Illumina Hotspot Design

Configure an AmpliSeq for Illumina Hotspot Design

1. Select a Sample Type.
Regular
FFPE
cfDNA

NOTE

DNA from high-quality FFPE tissue can be used with longer amplicon designs. However, uniformity and representation of longer amplicons can decrease.

2. Select the Max Amplicon Length.

375 bp is recommended for MiSeq only.

3. Select Next.