Reformat and Import Target Regions Into an AmpliSeq for Illumina Design
1. | Open the TruSeq Custom Amplicon design target regions file (*.bed) in a spreadsheet editor, such as Excel. |
2. | Delete the fifth and sixth columns from the file. |
3. | Make sure that all target names are 20 alphanumeric characters or less. Hyphens, periods, underscores, and whitespaces are allowed. |
The name field can be empty.
4. | Save and close the target regions file. |
5. | Upload the TruSeq Custom Amplicon design target regions file into the AmpliSeq for Illumina design. See Add Targets by Uploading Files. |