Reformat and Upload a Coordinates File
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1.
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Open the original TruSeq Custom Amplicon design coordinate input file (*.csv) in a spreadsheet editor, such as Excel. |
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2.
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Delete the following columns: |
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3.
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Rename the StartCoordinate column into Start. |
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4.
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Rename the StopCoordinate column into End. |
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5.
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Insert a column to the left of the Name column and enter Type in the header. |
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6.
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Enter REGION for all entries in the Type column. |
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7.
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Make sure that all entries in the Chromosome column include prefixes that match the chromosome format of the reference genome. See Expected Chromosome Format. |
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8.
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Make sure that all entries in the Name column are 20 alphanumeric characters or less. Hyphens, periods, underscores, and whitespaces are allowed. |
The name field can be empty.
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9.
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Save and close the coordinate input file. |
(missing or bad snippet)