Review a Completed Design
| 1. | Review the completed design summary in the top pane of the page. |
In addition to the options selected when creating and configuring the design, the following information is displayed:
|
Field |
Description |
|---|---|
|
Solution ID |
A unique identification number for the design automatically generated by DesignStudio. |
|
Assay Version |
The type of AmpliSeq for Illumina assay selected when creating the design. |
|
Species |
The species selected when configuring the design. |
|
Sample Type |
The type of sample selected when configuring the design. |
|
Max Amplicon Length |
The maximum amplicon length in the final design. |
|
Stringency |
The stringency selected when configuring the design. |
|
Number of Amplicons |
The number of amplicons included in the final design. |
|
Total target size (bp) |
The number of bases in the submitted targets. |
|
Covered Bases (bp) |
The number of bases covered by the custom amplicons. |
|
Number of Pools |
The number of pools included in the final design. |
|
Amplicons per Pool |
The number of amplicons included in each pool. |
|
Amplicon Length |
The maximum, minimum, average, and median length of amplicons (in base pairs) in the design. |
|
Percentage Coverage |
The percent of target bases covered by the custom amplicons. |
|
Concentration |
The concentration at which the manufactured amplicons are produced. |
| 2. | View detailed information on regions, amplicons, and gaps using the appropriate tabs in the table below the Review Design pane: |
| • | View Region Information |
| • | View Amplicon Information |
| • | View Gap Information |
| 3. | [Optional] Select Modify Design to create a project based on the current design. See Modify the Design. |
| 4. | [Optional] Select Download Targets to export the design targets in a CSV file. |
| 5. | [Optional] Select Download Results to download the design solution in a ZIP file. |
The design solution file contains all the design information generated from the project.
| 6. | [Optional] Select Download Amplicons to download the amplicons from the current design solution. |
The amplicon file is compatible with the upload tool on the Manage Targets page.
| 7. | [Optional] Select See more solutions to view and select additional solutions that improve coverage over the current design. |
The additional solutions include different combinations of stringency and number of pools that achieve different levels of coverage. When a solution is selected, it replaces all the selections made in the original design.
| 8. | To order a design, complete the following procedure. Sometimes, a completed design is not orderable. For unorderable designs, the following options are not available and DesignStudio displays a message with information on why the design cannot be ordered. |
- When ready to place an order, select Get Pricing.
- Review the final design details, and then select Finalize Design & Get Pricing. DesignStudio will redirect you to MyIllumina™ where you can purchase the custom assay.