Workflow Requirements

The minimum read length is 50 bases.
A minimum number of reads is not required. However, use sufficient data for each sample to support an appropriate depth of coverage for variant calling.
Each analysis uses only one target region BED.
Samples are all paired-end or all single-end.
A maximum of 96 samples is possible per analysis.
A maximum of 48 BAM or CRAM files is possible per analysis.