Current Limitations
Before running the TruSeq Phasing Analysis app, be aware of the following limitations:
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Only reads generated using the TruSeq Synthetic Long-Read Library Prep Kit can be analyzed with this app. |
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The short read length lower limit is 2×100 bp. The app supports the longest read lengths supported by the sequencing chemistry. |
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If you use a VCF file from a whole-genome sequencing analysis done outside of BaseSpace, the file must be from a whole-genome sequencing run with > 30× coverage for optimal results. See Input VCF Requirements for a description. |
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Currently, only diploid human genomes are supported. |
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Cancer samples are currently not supported. |
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If you have less than 30 Gbp in sequence data, you have insufficient data for optimal results. The recommended upper limit is 115 Gbp. |
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If there are < 1000 heterozygous SNVs in a single chromosome the TruSeq Phasing Analysis app exits. |
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The app restricts input to the first 1.5 million reads per barcode. |
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