Home/
Introduction/Workflow

Workflow

Global Expression Analysis—Gene-expression profiles generated by the RNA-Seq Alignment app using Salmon, are aggregated across all the samples at the transcript and gene levels. Principal component analysis is performed on all the samples.
Differential Expression Analysis—When differential expression analysis between the control and comparison groups is requested, DESeq2 is used to perform the analysis and hierarchical clustering is performed on samples across the two groups.