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Introduction | Workflow Requirements

Workflow Requirements

• Supports Illumina TruSight Tumor 170 assay
• Compatible with NextSeq or HiSeq 2500 Rapid Run systems
• Sequencing settings:
• 2 x 101 (recommended)
• Paired End (required)
• Connectivity to send sequencing run output data to BaseSpace Sequence Hub; it is recommended to stream run data directly to BaseSpace from the sequencing instrument
• Additional specifications can be found in the TruSight Tumor 170 data sheet
• Sample IDs on sample sheets configured using the Sample Sheet Template or the BaseSpace® Sequence Hub Prep Tab must meet the following criteria:
• < 60 characters
• Alphanumeric, underscore, and dash characters only
• No duplicate Sample ID
• Underscores and dashes must be preceded and followed by an alphanumeric character (no strings of multiple underscores or dashes, sample names cannot begin or end with an underscore or dash)
• Sequencing Run Setup
• Optional for NextSeq, Import CSV template for BaseSpace® Sequence Hub Prep Tab
• Required for HiSeq 2500 Rapid Run, Sample Sheet Template
• For DNA analysis
• Minimum of 80 M reads (40 M sequencing clusters) is recommended
• Paired-end samples with the same read lengths is required
• For RNA analysis
• Minimum of 16 M reads is recommended
• Paired-end samples with the same read lengths are required

For Research Use Only. Not for use in diagnostic procedures. 

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