Indexing QC
The Indexing QC page lists count information for indices used in the run. Note that the Indexing QC will only be available if the run is an index run.
You can select the displayed lane through the dropdown list.
The first table provides an overall summary of the indexing performance for that lane, including:
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Total Reads—The total number of reads for this lane. |
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PF Reads—The total number of passing filter reads for this lane. |
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% Reads Identified (PF)—The total fraction of passing filter reads assigned to an index. |
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CV—The coefficient of variation for the number of counts across all indices. |
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Min—The lowest representation for any index. |
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Max—The highest representation for any index. |
Further information is provided regarding the frequency of individual indices in both table and graph form. The table contains several columns, including
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Index Number—A unique number assigned to each index by BaseSpace for display purposes. |
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Sample ID—The sample ID assigned to an index in the sample sheet. |
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Project—The project assigned to an index in the sample sheet. |
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Index 1 (I7)—The sequence for the first index read. |
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Index 2 (I5)—The sequence for the second index read. |
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% Reads Identified (PF)—The number of reads (in terms of % Passing Filter reads) mapped to this index. |
This information is also displayed in graphical form. In the graphical display, indices are ordered according to the unique Index Number assigned by BaseSpace.
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