Sample Sheet

The sample sheet is a comma-delimited file (SampleSheet.csv) that stores much of the information needed to set up and analyze a sequencing experiment. The file includes a list of samples and their index sequences. Sample number zero is reserved for those clusters for which an index could not be identified. If the run does not have index read(s), all reads are given a sample number of 1.

The following table is for reference purposes only. For details about creating or modifying a sample sheet, see the MiSeq Sample Sheet Quick Reference Guide. You can create a sample sheet using the Illumina Experiment Manager.

Table:   Sample Sheet Fields

Row

Description

Date

The date the sequencing run was performed.

Data

Contaminants – The path to the folder containing FASTA files of contaminants (used only for SmallRNA)
GenomePath – The reference genome folder containing the FASTA files to be used in the alignment step
Index2 – Represents the sequence string of this sample's index. Valid characters in this string are A,C,G,T and N. 'N' matches any base.
IndexSequence – Represents the sequence string of a sample's index. Valid characters in this string are A, C, G, T and N. 'N' matches any base.
MiRNA – The path to the folder containing FASTA files of mature miRNAs (used only for SmallRNA)
RNA – The path to the folder containing FASTA files of small RNAs (used only for SmallRNA)
SampleID – A string identifier for the sample. This is usually a bar code but can have any value.
Manifest – The manifest file
Name – A string identifier for the sample. This is used in the reporting web page.

Experiment Name

(Optional) A descriptive name of the experiment.

Investigator Name

(Optional) The name of the investigator.

Manifests

This section is only used by the Amplicon workflow and is the name of the file (provided by Illumina) used in the Amplicon Workflow. It is mandatory for the Amplicon workflow and ignored by other workflows. The file specifies the alignments to a reference and the targeted reference regions used in the Amplicon workflow

Project Name

(Optional) A descriptive name of the run.

Site Reports

This section is optional and used by only the Resequencing and Custom Amplicon workflows. Each line below the SiteReports section header is the name of a SiteReport Input File. This file designates positions on a given chromosome to report the genotype found at that position.

Workflow

The analysis workflow for the run.

 

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Rev. August 20, 2012