M Bias (*.M-bias.txt)

The MethylSeq v1.0 app provides an M-Bias Report in a tab-delimited text (TXT) file format (*.M-bias.txt). The M-Bias Report describes the methylation proportion across each possible position in the read. The report contains a numerical value for M-Bias plot that can indicate the presence of fundamental technical biases in the methylation calling of reads. See Bismark.

Statistic

Definition

Position

The position of the C base.

Count methylated

The number of C bases that are methylated.

Count unmethylated

The number of C bases that are unmethylated.

% methylation

The percentage of methylation at that position.

Coverage

The total number of C bases at that position.

 

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