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Target Hits File Format

The TargetHitsPerSample_M1.tsv file is a tab-delimited file that contains the raw aligned replicate counts for each transcript.

The header of the file contains one row of column headings including sample IDs, followed by a row of sample names. For differential analysis, there must be at least two distinct sample names in the sample sheet.

This table describes the fields in the Target Hits file.

Column Heading	Description
Gene Name	The name of the gene.
Amplicon ID	The amplicon identifier constructed from the gene names, transcript IDs, exons, and target ID.
Target ID	The unique identifier for the target.
Transcript	The transcript ID(s) of the targeted amplicon.
Exons	The exons of the targeted amplicon.
Off Target	TRUE if the entry is a predicted off-target. FALSE otherwise.
Target Type	The type of this amplicon target.
Group Name	The group for each Sample ID.
Sample ID	Aligned count for all transcripts for this sample. There is one column for each sample using this manifest.

For Research Use Only. Not for use in diagnostic procedures.