Variant Call Files

Variant Call File (VCF) is a widely used file format developed by the genomics scientific community that contains information about variants found at specific positions in a reference genome.

VCF files use the file naming format SampleName_S#.vcf, where # is the sample number determined by the order that samples are listed in the sample sheet.

} VCF File Header—Includes the VCF file format version and the variant caller version. The header lists the annotations used in the remainder of the file. The VCF header also lists the reference genome. The last line in the header is column headings for the data lines. For more information, see VCF File Annotations.
} VCF File Data Lines—Contains information about a single variant. Data lines are listed under the column headings included in the header.

The TruSight Tumor 15 v1.0 App generates variant call files as VCF files and genome VCF files:

} VCF files contain information about variants found at specific positions.
} gVCF files contain information about all sites within the region of interest.

 

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