Prep Libraries

1 From the Libraries page, click the Library Prep Kit drop-down arrow and select a kit.

Note

If your kit is not listed but is compatible with your sequencing system, set up a custom library prep kit or select a kit that uses the same index adapter set. For example, select Nextera Rapid Capture for TruSight Cardio.

2 In the Plate ID field, enter a unique plate ID.
3 [Optional] In the Notes field, enter any notes. For Nextera Rapid Capture libraries, specify that the plate contains multiple enrichments.
4 Select the checkbox of each library to assign to a project.
5 Click Set Project.
6 In the Select Project(s) dialog box, click a project to assign libraries to, and then click Confirm.
7 If the Index By Well option is visible, select ON or OFF.
8 Click Auto Prep to assign each library is assigned to a well and index location. For Nextera Rapid Capture libraries, samples belonging to the same enrichment must be in the same row.

Note

Biological samples are assigned an index even if you are not performing indexed sequencing. During run setup, you can specify no indexing.

9 [Optional] Change indexes by clicking an index on the plate and selecting a new index from the drop-down list.

10 [Optional] Move libraries to a different well as follows.
    a Select the checkbox of the library to move.
    b Drag the selected library from the Libraries area to the appropriate well.
11 Click Export to save your library prep settings in a CSV file.

This file serves as a reference when preparing samples in the lab.

12 [Optional] Select additional plates as follows.
    a Select Save and Continue Later to return to Libraries.
    b Select the checkbox of each library you want to use.
13 Click Pool Libraries.