Review a Completed Design
| 1. | Review the completed design summary in the top pane of the page. |
If Spike-In targets were included in the design, On-Demand and Spike-In target details are shown separately. In addition to the options selected when creating the design, the following information is displayed:
|
Field |
Description |
|---|---|
|
Solution ID |
A unique identification number for the design automatically generated by DesignStudio. |
|
Assay Version |
The type of AmpliSeq for Illumina assay selected when creating the design. |
|
Species |
The species selected when configuring the design. |
|
Sample Type |
The type of sample selected when configuring the design. |
|
Max Amplicon Length |
The maximum amplicon length in the final design. |
|
Stringency |
The stringency in the design. For Spike-In targets, the stringency is always set to high. |
|
Number of Amplicons |
The number of amplicons included in the final design. |
|
Total target size (bp) |
The number of bases in the submitted targets. |
|
Covered Bases (bp) |
The number of bases covered by the custom amplicons. |
|
Number of Pools |
The number of pools included in the final design. |
|
Amplicons per Pool |
The number of amplicons included in each pool. |
|
Amplicon Length |
A stacked bar chart displaying maximum, minimum, average, and median length of amplicons (in base pairs) in the design. Appears as a list rather than a stacked bar chart if the difference between the minimum and maximum amplicon length is less than 6 bp. |
|
Percentage Coverage |
The percent of target bases covered by the Spike-In panel. |
| 2. | View detailed target information in the table below the Review Design pane. |
| 3. | [Optional] Select Modify Design to create a project based on the current design. See Modify the Design. |
| 4. | [Optional] Select Download Targets to export the design targets in a CSV file. |
| 5. | To order a design, complete the following procedure. |
Sometimes, a completed design is not orderable. For unorderable designs, the following options are not available and DesignStudio displays a message with information on why the design cannot be ordered.
- When ready to place an order, select Get Pricing.
- Review the final design details, and then select Finalize Design & Get Pricing. DesignStudio will redirect you to MyIllumina™ where you can purchase the custom assay.