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Create an AmpliSeq for Illumina Hotspot Design/Add Targets to the Design/Add Targets by Uploading Files

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Add Targets by Uploading Files

1. Select the File tab.
2. Select a File Format:
• csv—Comma-separated values (*.csv) files include five columns:

Column Header

Description

Type

Possible values are Snp or REGION

Name
• If type is set to REGION, this field holds a name to identify the chromosome region. Names should be 20 alphanumeric characters or less. Hyphens, periods, underscores, and whitespaces are allowed.
• If type is set to Snp, this field holds the variant name.

Chromosome

chromosome number. See Expected Chromosome Format.

Start

start position base pair coordinate

End

stop position base pair coordinate
• bed—Browser Extensible Data (*.bed) files include four columns:

Column

Description

chr

chromosome number. See Expected Chromosome Format.

start

start position base pair coordinate

stop

stop position base pair coordinate

[Optional] name

name to identify the chromosome region
3. [Optional] Select the download button next to the appropriate file format name to download a file template and enter gene or coordinate data in place of the sample entries.

When using a template, do not change column headings. Follow the format of the sample entries included in the template.

4. Select Select a File and navigate to the appropriate file.
5. Select Upload File.

DesignStudio validates the file and displays error messages for any entries that contain errors. After all the errors are fixed, return to step 4 to reimport the file.