Review a Completed Design

1. Review the completed design summary in the top pane of the page.

In addition to the options selected when creating the design, the following information is displayed:

Field

Description

Design Name

Project name entered when creating the design.

Design ID

A unique ID automatically assigned to each design by DesignStudio.

Assay Version

The type of AmpliSeq for Illumina assay selected when creating the design.

Species

The species selected when configuring the design.

Solution ID

A unique identification number for the design automatically generated by DesignStudio.

Max Amplicon Length

The maximum amplicon length in the final design.

Number of Amplicons

The number of amplicons included in the final design.

Number of Pools

The number of pools included in the final design.

Amplicon Length

A stacked bar chart displaying maximum, minimum, average, and median length of amplicons in the design. Appears as a list rather than a stacked bar chart if the difference between the minimum and maximum amplicon length is less than 6 bp.

2. View detailed target information in the table below the Review Design pane. The following information is displayed:

Heading

Description

Type

The target type.

Name

The gene name or transcript ID (when the row is expanded).

Symbol

The gene symbol.

Total

The number of transcripts for each gene.

Designed

The number of transcripts that are detectable by the designed primers. Transcript compatibility is displayed when the row is expanded.

3. [Optional] Select Modify Design to create a project based on the current design. See Modify the Design.
4. [Optional] Select Download Targets to export the design targets in a CSV file.
5. To order a design, complete the following procedure.

Sometimes, a completed design is not orderable. For unorderable designs, the following options are not available and DesignStudio displays a message with information on why the design cannot be ordered.

  1. When ready to place an order, select Get Pricing.
  2. Review the final design details, and then select Finalize Design & Get Pricing. DesignStudio will redirect you to MyIllumina™ where you can purchase the custom assay.