Cycle Plot

Cycle Plot

You can monitor the following quality metrics with this plot:

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Intensity—This plot displays the intensity by color of the 90% percentile of the data for each cycle.

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FWHM—The average full width of clusters at half maximum (in pixels).

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Corrected Intensity—The intensity corrected for cross-talk between the bases and phasing and prephasing.

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Called Intensity—The intensity for the called base.

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% Base—The percentage of clusters for which the selected base has been called.

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Error Rate—The calculated error rate, as determined by a spiked in PhiX control sample. If no PhiX control sample is run in the lane, this chart is not available.

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%Q>30—The percentage of bases with a quality score of 30 or higher, respectively. This chart is generated after the 25th cycle, and the values represent the current cycle.