Lane Plot

Lane Plot

The plots share a number of characteristics.

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The red line indicates the median tile value.

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The box outlines the interquartile range (the middle 50% of the data) for the tiles analyzed for the data point.

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The error bars delineate the minimum and maximum without outliers.

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The outliers are the values that are more than 1.5 times the interquartile range below the 25th percentile, or more than 1.5 times the interquartile range above the 75th percentile. Outliers are indicated as dots.

You can monitor the following quality metrics with this plot:

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Cluster Density—The number of clusters for each tile (k/mm2). The blue boxes are for raw clusters, the green boxes for clusters passing filter.

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% Phasing, % Prephasing—The percentage of molecules in a cluster for which sequencing falls behind (phasing) or jumps ahead (prephasing) the current cycle within a read. The graphs are split out by read.

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% Aligned—The percentage of the sample that aligned to the PhiX genome. The graphs are split out by read.