Requeue Analysis
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It is possible to requeue analysis from the MiSeq Reporter web interface. Before proceeding, check that a sequencing run is not in progress.
Each time analysis is requeued, the following folders and files are created:
• | A new Alignment folder is created in the MiSeqAnalysis folder with a sequential number appended to the folder name, such as Alignment2, for example. MiSeqAnalysis\<RunFolderName>\Data\Intensities\BaseCalls\Alignment2 |
• | Existing intermediate analysis files written in FASTQ file format are overwritten with new analysis files. FASTQ files are written to the BaseCalls folder. MiSeqAnalysis\<RunFolderName>\Data\Intensities\BaseCalls. |
Note
If changes were made to the sample sheet, make sure that a copy is saved to the root level of the analysis folder and the file is named SampleSheet.csv.
1 | From the MiSeq Reporter web interface, click Analyses. |
2 | Locate the run from the list of available runs on the Analyses tab, and click the Requeue checkbox adjacent to the run name. |
If the run is not listed, confirm that the correct repository is specified using the Settings icon. For more information, see Server URL or Repository Settings.
3 | Click Requeue. The State icon to the left of the run name changes to show that analysis is in progress ![]() |
• | If analysis does not start, make sure that the following input files are present in the analysis run folder: SampleSheet.csv, RTAComplete.txt, and RunInfo.xml. |
• | During analysis, a status bar and elapsed time appear on the Analysis Info tab. To stop analysis, select the stop analysis ![]() |
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