HiSeq X FAQs

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  • General


  • The HiSeq X Ten is a collection of 10 ultra-high throughput HiSeq X Sequencing Systems. HiSeq X Ten can sequence more than 18,000 genomes per year at a price point of less than $1000 USD per genome.

    Each system can generate 1.6–1.8 Tb in less than 3 days with greater than 75% of bases about Q30 from a 2 x 150 bp run. This throughput enables 16 genomes covered at 30x per run per system. For more information, see HiSeq X Series Specifications.

  • Reagents and Flow Cells


  • The HiSeq X Reagent Kit v2.5 requires HiSeq X Control Software v3.1, or later.

    The HiSeq X system uses patterned flow cell technology. Reagents provided in the HiSeq X Reagent Kit are designed and optimized for the HiSeq X flow cell. Non-patterned flow cells and SBS kits designed for other HiSeq models have not been validated and are not supported for use on the HiSeq X system.

    No. HiSeq X reagents are designed and optimized for HiSeq X Systems. HiSeq X reagents are not compatible with other HiSeq models.

    A patterned flow cell has billions of ordered nanowells that are manufactured into the flow cell glass. The ordered wells allow for the generation of sequencing clusters in an ordered arrangement. Clusters are aligned more closely together, increasing the number of output reads and amount of sequence data generated.

    The HiSeq X patterned flow cell contains eight lanes and has the same general dimensions as a HiSeq high-output flow cell.

    No. HiSeq X flow cells are designed and optimized for use on the HiSeq X System. HiSeq X flow cells are not compatible with other HiSeq models.

    Each kit includes two flow cells and all required reagents, including clustering, SBS, paired-end, and indexing reagents. One kit provides consumables for a 150-cycle paired-end (2 ×150 bp) sequencing run plus indexing on both flow cell A and flow cell B.

    With the release of the HiSeq X Reagent Kit v2, a new kit configuration called the 10-pack kit is available. The 10-pack kit includes flow cells and consumables for 10 dual flow cell runs or 20 single flow cell runs. The 10-pack is designed to support the preparation and sequencing of 4 flow cells at a time, and significantly reduces storage requirements.

    The HiSeq X Reagent Kit v2 requires HiSeq X Control Software v3.1, or later.

  • Analysis


  • You can expect greater than 75% of bases above Q30 with a 2 x 150 bp run using the following libraries:

    • A human library prepared with the TruSeq Nano DNA Sample Prep Kit (350 bp or 450 bp insert)
    • A human library prepared with the TruSeq DNA PCR-Free Sample Prep Kit (350 bp or 450 bp insert)
    • Illumina PhiX control library