Questions & Answers

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Libraries

  • How many samples can I sequence at a time on the MiniSeq System?

    The MiniSeq flow cell is a single-lane flow cell, which requires that samples prepared for sequencing be combined in a single library pool. The library pool is loaded into a reservoir on the MiniSeq reagent cartridge before the run, and transferred onto the flow cell after the run is started.

    The number of samples that can be combined in a library pool depends on the library prep method. See the documentation for your library prep kit.

  • What applications can I run on the MiniSeq System?

    The MiniSeq System enables small genome, amplicon, targeted enrichment, and RNA sequencing of low library volumes with associated analysis options using BaseSpace Apps or Local Run Manager analysis modules. For more information, see the MiniSeq System applications page.

    For a list of specific library prep kits associated with Local Run Manager analysis modules, see Compatible Libraries on the Local Run Manager support page.

Workflow

  • How long does it take to perform a MiniSeq run?

    The duration of a sequencing run depends on the type of run and number of cycles performed. See the MiniSeq specifications page for more information.

Analysis

  • What is Local Run Manager?

    Local Run Manager is on-instrument analysis software integrated with the system control software. Using Local Run Manager, you can record sample information and specify run parameters before starting the run. The name assigned to the run appears on the control software run setup screen for faster run setup.

    When the sequencing run is complete, data analysis begins on-instrument automatically and performs analysis according to the analysis module specified for the run.

  • Can I use third-party analysis options with MiniSeq sequencing data?

    Yes. Third-party analysis options require sequencing data in the FASTQ file format. Use the Local Run Manager Generate FASTQ analysis module to convert data for later use. The Generate FASTQ analysis module converts base calls to FASTQ files and then exits the workflow. No further analysis is performed.