AmpliSeq for Illumina Cancer Hotspot Panel v2 FAQs

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  • Analysis

  • The DNA Amplicon analysis workflow can be used to perform alignment and variant calling.  Local Run Manager and BaseSpace Sequence Hub have apps available for analysis. The DNA Amplicon Analysis App is available on BaseSpace Sequence Hub. Further analysis can be performed on any variant calls using BaseSpace Variant Interpreter.  Local Run Manager has a similar DNA Amplicon Analysis Module which utilizes the same workflow and algorithm as the BaseSpace Sequence Hub Apps. 

    Yes, there are example data sets in BaseSpace Public Data.

    On-target bases shows the percentage of total sequenced bases that map to target regions in the reference genome. This metric reflects the percentage of bases from amplicons that a) were designed, synthesized, and pooled and b) generated sequence data mapping to the target regions.

    No. The hotspots are variants that have been tested and can be detected using the panels and the Illumina DNA Amplicon analysis. Other variants within the targeted region may be detected as well.