Contents & Storage

Clustering reagents are shipped in 2 boxes on dry ice. Each kit includes a patterned flow cell, a manifold, and the required reagents and accessories for clustering the flow cell on the cBot.

When you receive your kit, promptly store the kit components at the indicated temperature to ensure proper performance.

 

Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Reagent Plate

PE Kit—cBot reagent plate, PE

SR Kit—cBot reagent plate, SR

1

RSB

Resuspension Buffer

1

EPX1

Enhanced Pattern Cluster Mix 1

1

EPX2

Enhanced Pattern Cluster Mix 2

1

EPX3

Enhanced Pattern Cluster Mix 3

 

PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HRM

HT Resynthesis Mix

1

HLM2

HT Linearization Mix 2

1

HAM

HT Amplification Mix

1

HPM

HT Amp Premix

1

HDR

HT Denaturation Mix (contains formamide)

1

HP11

Primer Mix – Read 2

1

HP14

Indexing Primer Mix

 

SR Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HRM

HT Resynthesis Mix

1

HDR

HT Denaturation Mix
(contains formamide)

1

HP12

Index 1 Primer Mix

 

Accessory Kit

Quantity

Accessory

Purpose

1

Flow cell gaskets (package of 4)

Replace the flow cell gaskets between runs.

1

Flow cell storage tube with storage buffer, 50 ml

Store the flow cell between clustering and sequencing.

1

Funnel bottle caps (package of 8)

Replace the caps on SBS reagent bottles before loading reagents.

The HiSeq 3000/4000 Cluster Kit also includes the following:

  • Patterned Flow Cell (Quantity 1, Store at 2°C to 8°C)
    • PE Kit—Flow Cell, PE
    • SR Kit— Flow Cell, SR  
  • cBot Manifold (Quantity 1)