Requirements & Compatibility

Components

HiSeq v4 reagent kits are compatible with the HiSeq 2500, HiSeq 1500, and HiSeq 2000. Always use compatible kits and software versions.

Cluster Kit Version

SBS Kit Version

HCS/RTA Version

HiSeq Cluster Kit v4

HiSeq SBS Kit v4

HCS v2.2/RTA v1.18, or later

SBS Kits for Dual-Indexed Sequencing

HiSeq SBS kits contain a sufficient volume of reagents for performing dual-indexed runs:

  • One 250-cycle SBS kit provides reagents for up to 275 cycles of sequencing.
  • One 50-cycle kit provides reagents for up to 75 cycles of sequencing.
  • For runs greater than a total of 275 cycles, use multiple 50-cycle kits.

The following table lists the number of cycles performed for dual-indexed runs of various lengths. For dual-indexed paired-end runs, 7 chemistry-only cycles are calculated in the total number of cycles.

Run Type

Run Length

Run Cycles

Total Cycles

Dual-Indexed Paired-End

2 x 125

126+8+(7)+8+126

275

2 x 100

101+8+(7)+8+101

225

2 x 75

76+8+(7)+8+76

175

2 x 50

51+8+(7)+8+51

125

2 x 35

36+8+(7)+8+36

95

Dual-Indexed Single-Read

1 x 125

126+8+8

142

1 x 75

76+8+8

92

1 x 50

51+8+8

67

1 x 35

36+8+8

52