NextSeq 500/550 Kits

Catalog IDs: FC-404-1001, FC-404-1002, FC-404-1003, FC-404-1004, FC-404-1005

Overview

To perform a sequencing run on the NextSeq system, you need one single-use NextSeq 500/550 Kit. Each kit includes the flow cell, and clustering and sequencing reagents required for a sequencing run. The flow cell, reagent cartridge, and buffer cartridge use radio-frequency identification (RFID) for accurate consumable tracking and compatibility.

Kit Versions and Software Compatibility

NextSeq 500/550 Kit v2—Reagents provided in the NextSeq 500/550 Kit v2 in combination with NextSeq Control Software v1.4, or later, generate improved data quality and a higher yeild of error-free reads. Additionally, the NextSeq 500/550 Kit v2 provides an improved workflow during run setup and enables the use of a custom Index 2 primer.

NextSeq 500 Kit v1—The NextSeq 500 Kit v1 requires NextSeq Control Software v1.3, or earlier. This kit is not compatible with later versions of the control software.
Using this kit requires additional run set up steps, which includes the addition of NaOCl and BP13 to the reagent cartridge. See Additional Setup Step for NextSeq 500 Kit v1.
The NextSeq 500/550 Kit v2 includes these reagents in the prefilled cartridge. No additional steps are required.

Version Compatibility—Each kit version requires a compatible version of control software.

Kit Types and Sizes

  • The NextSeq 500/550 High Output Kit includes a high-output flow cell and compatible reagent cartridge. Each component is labeled High.
  • The NextSeq 500/550 Mid Output Kit includes a mid-output flow cell and compatible reagent cartridge. Each component is labeled Mid.

Kit Type

Kit Sizes

NextSeq 500/550 High Output Kit

300 cycles, 150 cycles, and 75 cycles

NextSeq 500/550 Mid Output Kit

300 cycles and 150 cycles

  • 300 cycles—Provides reagents for up to 300 cycles (2 x 150 cycles) of sequencing and 2 index reads of 8 cycles each.
  • 150 cycles—Provides reagents for up to 150 cycles (2 x 75 cycles) of sequencing and 2 index reads of 8 cycles each.
  • 75 cycles—Provides reagents for up to 75 cycles (1 x 75 cycles) of sequencing and 2 index reads of 8 cycles each.

Kit Documentation

For information about NextSeq 500/550 kits, see Contents & Storage and Requirements & Compatibility. For preparation instructions, see the  NextSeq 500 System Guide or the NextSeq 550 System Guide.

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