Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)

Catalog IDs: MRZG126, MRZG12324

The Ribo-Zero Gold rNA Removal Kit (Human/Mouse/Rat) will be discontinued on November 2nd, 2018. The TruSeq Stranded Total RNA Library Prep Gold is the recommended replacement product. Illumina remains committed to providing customers with high-quality support and service.

Product Description

The Ribo-Zero Gold (Human/Mouse/Rat) kit is compatible with human and other animal species, including FFPE RNA samples. This kit removes eukaryotic cytoplasmic rRNA and mitochondrial rRNA.

Performance Specifications

Compare rRNA content before and after a Ribo-Zero reaction to assess the depletion of rRNA in total RNA.

A Ribo-Zero reaction removes:

  • > 99% of large cytoplasmic rRNA and large mitochondrial and chloroplast rRNA, as applicable.
  • > 85% to 95% of small rRNA (eg, 5S).

The success of rRNA removal depends on factors such as species, sample quality, and sequence homology between the rRNA sample and removal solution, especially with 5S rRNA removal. Use the Epicentre RNAMatchMaker at epibio.com/rnamatchmaker to verify sample and kit sequence homology sequence homology.

Input Requirements

This protocol supports rRNA depletion from 1–5 µg total RNA samples. Use a fluorometric-based method to quantify input.