Contents & Storage

Contents & Storage

Clustering reagents are shipped in multiple boxes on dry ice. Each kit includes a flow cell, a manifold, and the required reagents and accessories for clustering the flow cell on the cBot.

When you receive your kit, promptly store the kit components at the indicated temperature to ensure proper performance.

 

PE Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HP3

Denaturation Solution

1

HT2

Wash Buffer

1

RMR

Resynthesis Mix

1

AT2

100% Formamide

1

APM2

Amplification Premix

1

AMX2

Amplification Mix 2

1

LMX2

Linearization Mix 2

1

BMX

Blocking Mix

1

HP7*

Read 2 Sequencing Primer Mix

* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.

 

PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Kit Component

Description

1

cBot Reagent Plate

cBot PE reagent plate

1

HT1

Hybridization Buffer

 

SR Cluster Kit Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Reagent Plate

cBot SR reagent plate

1

HT1

Hybridization Buffer

 

Multiplex Sequencing Primer Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HP3

Denaturation Solution

1

HT2

Wash Buffer

1

HP8*

Index Sequencing Primer

* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.

 

Accessory Kit

Quantity

Component

Purpose

4

Flow cell gaskets

Replace the flow cell gaskets between runs.

8

Funnel bottle caps

Replace SBS reagent bottle caps before loading reagents.

1

ICB instrument tube

Divide ICB for Read 2.