TruSeq DNA Nano FAQs

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  • General


  • Illumina recommends using Coriell Human-1 DNA (NA18507) or Promega Human Genomic DNA (G3041) as a positive control for this protocol.

    The key advantage to using TruSeq DNA Nano is that only 100 ng of starting genomic DNA is required. This is increasingly important in studies that have limited amounts of DNA or where DNA needs to be split into multiple applications. In addition, TruSeq DNA Nano is a comprehensive solution containing all of the necessary reagents, barcodes, and size selection beads for convenient processing. The improved workflow includes bead-based size selection, therefore eliminating the time of gel-based methods. TruSeq DNA Nano also produces premier library quality, offering an excellent solution for studies requiring the highest coverage.

    The polymerases used in the kits are not appropriate for bisulfite applications.

    We offer two kits to support the following sample numbers.

    Library Prep Component

    Catalog #

    TruSeq Nano (24 samples)

    20015964

    TruSeq Nano (96 samples)

    20015965

    The following library prep and index adapter components are available to order through Illumina. From Illumina, order one catalog number for the library prep component and one catalog number for the index adapter component depending on the number of samples for your experiment.

    Library Prep Component

    Catalog #

    TruSeq Nano (24 samples)

    20015964

    TruSeq Nano (96 samples)

    20015965

     

     

    Index Adapter Option

     

    IDT for Illumina- TruSeq DNA UD Indexes (24 indexes)

    20020590

    IDT for Illumina- TruSeq DNA UD Indexes (96 indexes)

    20020591

    TruSeq DNA CD Indexes- 96 Indexes

    20015949

    TruSeq DNA Single Index Set A- 12 Indexes

    20015960

    TruSeq DNA Single Index Set B- 12 Indexes

    20015961

    Single index adapters are provided in tubes.  Each i7 indexed adapter is paired in its tube with the same universal adapter that contains no (i5) barcode.

    Combinatorial Dual Indexed Adapters are plated pairs of adapters such that every i5 indexed adapter would be paired with each i7 indexed adapter in a arrayed matrix across the plate.

    Unique Dual Indexed Adapters are plated pairs of adapters such that every i5 indexed adapter and every i7 indexed adapter is only used once on a plate. With unique dual index adapters, identification and filtering of indexed hopped reads allow customers to confidently multiplex their samples.

    Illumina recommends using Unique Dual Indexed Adapters for library prep and sequencing on Illumina sequencers.

    No, this kit requires high quality gDNA as starting material. Use of degraded DNA may result in low yields and loss of sample during bead clean-up steps.

    No, Sample Purification Beads (SPB) that are used for size-selection and clean-up steps are included. 

  • Input


  • The protocol has been developed and validated with 100 ng of high quality DNA for a 350 bp insert size and 200 ng high quality DNA for a 550 bp insert size.