This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
After completing this course you will be able to describe the TruSeq Targeted RNA Expression Kit, the steps to order a custom oligo pool, the steps in the TruSeq Targeted RNA Expression protocol, best practices, and how to analyze and normalize data.
After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.
Recorded Webinar (March 2020) | Have a targeted sequencing project in mind? This webinar is targeted toward new and intermediate users looking to design a custom targeted sequencing assay in DesignStudio. We wiill show you how to use the software, and share tips and tricks to create a design for AmpliSeq for Illumina, Nextera Rapid Capture, and TruSeq Targeted RNA Expression.
Recorded Webinar (July 2019) | Part 1 of a 4-Part RNA-Seq Webinar Series. This session describes key considerations when planning RNA sequencing experiments. We will cover RNA sample preparation through sequencing to basic data analysis. We assume a basic understanding of Illumina sequencing technology, but no prior knowledge of RNA sequencing is required. The webinar is targeted towards both new and experienced users.
Recorded Webinar (August 2019) | Part 2 of a 4-part RNA-Seq webinar series. This session will review sample preparation kit options for total RNA, mRNA, and targeted RNA sequencing projects. Great for both new and experienced users, we will assume a basic understanding of Illumina sequencing technology, although no knowledge of sample prep is required. In particular, we’ll discuss: how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.
Recorded Webinar (August 2019) | Part 3 of a 4-part RNA-Seq webinar series. In the third part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s TruSeq RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, and TruSeq Stranded Total RNA and discuss: Bead handling, quantification methods, library QC, and troubleshooting.
Upcoming Webinar | Part 1 of a 3-Part RNA-Seq Webinar Series. This session will review sample preparation kit options for RNA input. An introduction to the new Illumina Stranded mRNA Ligation, Illumina Stranded Total RNA Ligation with Ribozero Plus, and Illumina RNA prep with Enrichment Tagmentation workflows will be provided along with a comparison to established Illumina kits. This webinar is targeted towards both new and experienced users and will cover the following topics: key considerations when planning RNA sequencing experiments, how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.
Oct 13, 2020 PST