Requirements & Compatibility

Component Compatibility

TruSeq v3 reagent kits are compatible with the HiSeq 2500, HiSeq 1500, and HiSeq 2000. Always use compatible kits and software versions.

Cluster Kit Version

SBS Kit Version

HCS/RTA Version

TruSeq Cluster Kit v3

TruSeq SBS Kit v3

HCS v1.4 / RTA v1.12, or later

SBS Kits for Dual-Indexed Sequencing

The following table lists the number of cycles performed for dual-indexed runs of various lengths. For dual-indexed paired-end runs, 7 chemistry-only cycles are calculated in the total number of cycles.

  • One 200-cycle kit provides sufficient reagents for up to 209 cycles of sequencing.
  • One 50-cycle kit provides sufficient reagents for 58 cycles of sequencing.
  • For runs greater than a total of 209 cycles, and to maximize reagent use for other read lengths, use multiple 50-cycle kits.

Run Type

Run Length

Run Cycles

Total Cycles

Dual-Indexed Paired-End

2 x 100

101+8+(7)+8+101

225

2 x 92

93+8+(7)+8+93

209

2 x 75

76+8+(7)+8+76

175

2 x 50

51+8+(7)+8+51

125

2 x 35

36+8+(7)+8+36

95

Dual-Indexed Single-Read

1 x 100

101+8+8

117

1 x 75

76+8+8

92

1 x 50

51+8+8

67

1 x 35

36+8+8

52