Catalog IDs: 20000215, 20005170
The TruSight HLA v2 Sequencing Panel protocol explains how to prepare indexed libraries from input DNA for subsequent cluster generation and DNA sequencing using the reagents provided in the Illumina TruSight HLA v2 Sequencing Panel. The goal of the protocol is to isolate and amplify HLA loci with a long-range polymerase chain reaction (PCR). After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate DNA libraries ready for sequencing on the Illumina MiSeq systems.
The TruSight HLA v2 Sequencing Panel protocol provides:
The protocol describes how to process 12 samples in parallel. The TruSight HLA v2 Sequencing Panel kit includes enough reagents to process 24 samples in total.