Off-Line Basecaller (OLB)

OLB Overview

The Off-Line Basecaller (OLB) performs base calling and bcl to qseq conversion for the HiSeq, HiScan-SQ, or Genome Analyzer.

The standard workflow is to perform base calling using on-instrument real time analysis (RTA), after which CASAVA performs alignment using the base calling results. If needed, OLB provides the option to perform primary data analysis off-line. In addition, OLB allows you to convert per-cycle base call files (*.bcl) into per read base call files (*_qseq.txt).