How many cycles of SBS chemistry are in my kit?

07/27/18


Making sure that sufficient volumes of SBS reagents are loaded onto the sequencing instrument is crucial for a successful run. Running out of reagents during a run impacts intensity and quality, and can result in a run failure. On certain instruments, dual-index runs need an additional 7 cycles of reagents for the chemistry-only cycles at the beginning of the second Index Read. These 7 cycles must be considered when calculating the number of cycles supported by the kit.

The following table outlines available reagent kit sizes and the maximum number of cycles each kit can perform:

Reagent Type Reagent Kit Size Maximum Number of Cycles Single-Indexed Example
HiSeq - TruSeq SBS v3 50 58 7 cycles required for paired-end flow cells
200 209
HiSeq - HiSeq SBS v4 50 75
250 275
HiSeq - Rapid SBS v2 50 75
200 225
500 525
MiSeq v2 (including Micro and Nano kits) 50 75 Yes - 7 cycles
300 325
500 525
MiSeq v3 150 175
600 625
NovaSeq 6000 S1 and S2 100 130
200 230
300 330
NovaSeq 6000 S4 300 330
HiSeq 3000/4000 SBS 50 75 7 cycles required for single-read flow cells
150 175
300 325
iSeq 100 i1 300 318 No
MiniSeq High Output or Mid Output 75 92
150 168
300 318
NextSeq 500/550 High Output or Mid Output v2 and v2.5 75 92
150 168
300 318

 

For more information about each SBS reagent kit, including how to prepare, combine, and split SBS reagents when possible, visit the support page for each kit.