Introduction

The BaseSpace Sequence Hub 16S Metagenomics app analyzes DNA from amplicon sequencing of prokaryotic 16S small subunit rRNA genes. The application performs read classification through a high performance version of the RDP Naïve Bayes taxonomic classification algorithm. Classification statistics are calculated, both by sample and in aggregate, and results are summarized in interactive visualizations. Raw classification data are also available for download.

The main output files generated by 16S Metagenomics are:

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Summary.csv files, containing summarized classification statistics for each sample.

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Txt.gz compressed files, containing per-read classifications for each sample.

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Aggregate_Counts.csv files, containing aggregate classification results by taxonomic level across all samples.

In addition, there are per-sample and aggregate PDF and HTML reports, as well as XML statistics files.

See 16S Metagenomics Methods and View Analysis Output for more information.