Set Analysis Parameters
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1.
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Open 16S Metagenomicsfrom BaseSpace Sequence Hub as follows. |
- Select the Apps tab, and then select 16S Metagenomics.
- From the Version drop-down list, select 1.1.0.
- Select Launch Application.
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2.
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To override the default analysis name, enter a preferred analysis name in the Analysis Name field. |
The default is the app name with the date and time the session started.
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3.
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From the Save Results To field, select Select Project, and then select a project to store app results in. |
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4.
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From the Biosample(s) field, select the biosample you want to analyze as follows. |
- Select Select Biosample(s).
- Select the checkbox of each biosample you want to analyze, and then select Select.
The labels of this field and button in the software interface might be different from the labels in this guide, depending on the BaseSpace Sequence Hub mode. In New mode, all data that is associated with the selected biosample is used in the analysis.
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5.
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From the Database drop-down list, select a taxonomy database to use for the analysis. |
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6.
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If you selected Custom from the Database drop-down list, select Select Dataset File(s) from the Custom Database field, and then select a custom taxonomy database file. |
Use only custom database files created with the 16S Metagenomics Database Creator app.
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7.
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Select Launch Application to start the analysis. |
When the analysis is complete, the status of the app session is automatically updated and you receive a confirmation email.