Workflow Requirements
• | The minimum read length is 50 bases. |
• | Each sample must have no more than 30 gigabases. |
• | A minimum number of reads is not required. However, use sufficient data for each sample to support an appropriate depth of coverage for variant calling. |
• | Each analysis uses only one manifest. |
• | Samples are all paired-end or all single-end. |
• | Sequenced samples have the same read lengths. |
• | A maximum of 384 samples is possible per analysis. |
• | AmpliSeq for Illumina DNA, RNA, and DNA + RNA Amplicon panels are supported. |
• | For RNA Amplicon, only gene expression panels are supported. For differential expression, use the RNA Amplicon app. |
• | Supports a maximum of 200 GB of sequence. |