Workflow Requirements
| • | The minimum read length is 50 bases. |
| • | Each sample must have no more than 30 gigabases. |
| • | A minimum number of reads is not required. However, use sufficient data for each sample to support an appropriate depth of coverage for variant calling. |
| • | Each analysis uses only one manifest. |
| • | Samples are all paired-end or all single-end. |
| • | Sequenced samples have the same read lengths. |
| • | A maximum of 384 samples is possible per analysis. |
| • | AmpliSeq for Illumina DNA, RNA, and DNA + RNA Amplicon panels are supported. |
| • | For RNA Amplicon, only gene expression panels are supported. For differential expression, use the RNA Amplicon app. |
| • | Supports a maximum of 200 GB of sequence. |