Amplicon Summary

Table 1   Amplicon Summary Table

Statistic

Definition

Manifest (PDF only)

The name of the manifest used in the analysis.

Number of Amplicon Regions

The number of amplicon regions that were sequenced.

Total Length of Amplicon Regions

The total length of the sequenced bases in the targeted region.

Table 2   Read Level Statistics Table

Statistic

Definition

Total Aligned Reads

The total number of reads passing filter present in the data set that aligned to the reference genome.

Percent Aligned Reads

The percentage of reads passing filter that aligned to the reference genome.

Table 3   Base Level Statistics Table

Statistic

Definition

Percent Q30

The percentage of bases with a quality score of 30 or higher.

Total Aligned Bases

The total number of bases present in the data set that aligned to the reference genome.

Percent Aligned Bases

The percentage of bases that aligned to the reference genome.

Mismatch Rate

The average percentage of mismatches across both reads 1 and 2 over all cycles.