DNA Sample Metrics Report

File name: DNA_SampleMetricsReport.txt

Review this report to assess DNA sample data quality using the following metrics.

Metric	Description	Passing Quality Threshold	Indication When Failed
Coverage_MAD	Median of absolute deviations from the median of the normalized count of each CNV target region	≤0.2	Less confidence in Copy Number Variant calling
Median_BinCount_CNV_Targets	Median of the raw read counts (normalized by region size) of each CNV target region	≥1	Less confidence in Copy Number Variant calling
Median_Insert_Size	Median of fragment sizes calculated from read alignments	≥79	Less confidence in Small Variant calling
PCT_ExonBases_100X	Percentage of exonic bases in gVCF that have greater than or equal to 100x coverage	≥0.95	Less confidence in small variant calling
PCT_Chimeric_Reads	Percentage of unique reads that are part of a chimeric alignment (ie, one half of the read maps to one location and the other half maps to a nonsequential position) out of total mapped unique reads	N/A	Not used to evaluate sample data quality

For Research Use Only. Not for use in diagnostic procedures.