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Set Analysis Parameters/Manifest Files

Manifest Files

A manifest file is required input for the Enrichment app. The manifest is a tab-delimited text (*.txt) file that specifies the names and locations of targeted reference regions. The main section of the manifest file is the Regions section and contains the following data columns:

Column

Description

Name

Unique user-specified name for the target

Chromosome

Chromosome location (eg, chr10, chr5, etc.)

Start

1-based index for the start position of the target

Stop

1-based index for the stop position of the target

Upstream Probe Length

The length of the upstream probe. For the Enrichment app, this should be set to 0.

Downstream Probe Length

The length of the downstream probe. For the Enrichment app, this should be set to 0.

For a manifest file template for the Enrichment app, visit the Downloads section of the BaseSpace Sequence Hub support page on the Illumina website.